GSE73969-GSM1906889-GPL21021-PMID_27173635PMID_28288207.tsv 6.43 KB
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	data_processing	Illumina Casava1.7 software used for basecalling. PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	data_processing	All raw FastQ files generated were filtered using FASTX-Toolkit (http://hannonlab.cshl.edu/fastx_toolkit/). PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	data_processing	Reads per kilobase of exon model per million mapped reads (RPKM) was used as a normalized metric to present the gene expression levels.(Nature methods 2008, 5: 621-628) PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	data_processing	Transcripts evaluation of differential expression were accomplished using MARS (MA-plot-based method with Random sampling model) in DEGseq package.(Bioinformatics 2010, 26: 136-138) PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	data_processing	Genome_build: NC_002655.2 PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	data_processing	Supplementary_files_format_and_content: RPKM PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	extract_protocol	RNA extraction was performed using RiboPure-BacteriaTM Bacteria kit according to the manufacturer[linebreak]s protocol (Ambion, Life Technologies). DNase I treatment was applied to eliminate genomic DNA contamination as per the manufacturer[linebreak]s recommendations. PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	extract_protocol	RNA libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	growth_protocol	Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an OD600 of about 0.8. PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	organism	Escherichia coli O157:H7 str. EDL933 PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	source_name	Enterohemorrhagic Escherichia coli O157:H7 strain EDL933 PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	treatment_protocol	The bacteria were centrifuged at 4000×g at 4°C for 5 min and then washed with PBS. PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	characteristics	genotype: {delta}hns PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	characteristics	phase: logarithmic phase PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	growth_protocol	Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an OD600 of about 0.8. PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	organism	Escherichia coli O157:H7 str. EDL933 PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	source_name	Enterohemorrhagic Escherichia coli O157:H7 strain EDL933 PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	treatment_protocol	The bacteria were centrifuged at 4000×g at 4°C for 5 min and then washed with PBS. PGCGROWTHCONDITIONS
SRR2637697	GSE73969	GSM1906889	GPL21021	PMID_27173635PMID_28288207	hns_1	RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933	GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933)	contact_name	Baoshan,,Wan PGCGROWTHCONDITIONS