GSE73969-GSM1906889-GPL21021-PMID_27173635PMID_28288207.tsv
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SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) data_processing Illumina Casava1.7 software used for basecalling. PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) data_processing All raw FastQ files generated were filtered using FASTX-Toolkit (http://hannonlab.cshl.edu/fastx_toolkit/). PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) data_processing Reads per kilobase of exon model per million mapped reads (RPKM) was used as a normalized metric to present the gene expression levels.(Nature methods 2008, 5: 621-628) PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) data_processing Transcripts evaluation of differential expression were accomplished using MARS (MA-plot-based method with Random sampling model) in DEGseq package.(Bioinformatics 2010, 26: 136-138) PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) data_processing Genome_build: NC_002655.2 PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) data_processing Supplementary_files_format_and_content: RPKM PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) extract_protocol RNA extraction was performed using RiboPure-BacteriaTM Bacteria kit according to the manufacturer[linebreak]s protocol (Ambion, Life Technologies). DNase I treatment was applied to eliminate genomic DNA contamination as per the manufacturer[linebreak]s recommendations. PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) extract_protocol RNA libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) growth_protocol Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an OD600 of about 0.8. PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) organism Escherichia coli O157:H7 str. EDL933 PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) source_name Enterohemorrhagic Escherichia coli O157:H7 strain EDL933 PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) treatment_protocol The bacteria were centrifuged at 4000×g at 4°C for 5 min and then washed with PBS. PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) characteristics genotype: {delta}hns PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) characteristics phase: logarithmic phase PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) growth_protocol Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an OD600 of about 0.8. PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) organism Escherichia coli O157:H7 str. EDL933 PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) source_name Enterohemorrhagic Escherichia coli O157:H7 strain EDL933 PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) treatment_protocol The bacteria were centrifuged at 4000×g at 4°C for 5 min and then washed with PBS. PGCGROWTHCONDITIONS
SRR2637697 GSE73969 GSM1906889 GPL21021 PMID_27173635PMID_28288207 hns_1 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) contact_name Baoshan,,Wan PGCGROWTHCONDITIONS