Set up directory

+The results of the present study extend these observations .

+that the MetR protein also stimulates the in-vitro expression of metH

+that both MetH proteins synthesized in-vitro are enzymatically active

+that both the MetE synthesized in-vitro are enzymatically active

+TYPE: sentence[0..226]
+TEXT: The results of the present study extend these observations and show that the MetR protein also stimulates the in-vitro expression of metH and that both the MetE and MetH proteins synthesized in-vitro are enzymatically active .
+POS : The_DT results_NNS of_IN the_DT present_JJ study_NN extend_VBP these_DT observations_NNS and_CC show_VBP that_IN the_DT MetR_NN protein_NN also_RB stimulates_VBZ the_DT in-vitro_FW expression_NN of_IN metH_NN and_CC that_IN both_CC the_DT MetE_NN and_CC MetH_NN proteins_NNS synthesized_VBN in-vitro_FW are_VBP enzymatically_RB active_JJ ._. 
+TREE: (ROOT (S (NP (NP (DT The) (NNS results)) (PP (IN of) (NP (DT the) (JJ present) (NN study)))) (VP (VP (VBP extend) (NP (DT these) (NNS observations))) (CC and) (VP (VBP show) (SBAR (SBAR (IN that) (S (NP (DT the) (NN MetR) (NN protein)) (ADVP (RB also)) (VP (VBZ stimulates) (NP (NP (DT the) (FW in-vitro) (NN expression)) (PP (IN of) (NP (NN metH))))))) (CC and) (SBAR (IN that) (S (NP (CC both) (NP (DT the) (NN MetE)) (CC and) (NP (NN MetH) (NNS proteins))) (VP (VBN synthesized) (S (NP (FW in-vitro)) (VP (VBP are) (ADJP (RB enzymatically) (JJ active)))))))))) (. .)))
+SIMP:
+  TYPE: noun or noun phrase coordination[152..178]
+  TEXT: the MetE and MetH proteins
+  COMP: conjunct[152..160]
+  COMP: conjunction[161..164]
+  COMP: conjunct[165..178]
+SIMP:
+  TYPE: verb or verb phrase coordination[33..224]
+  TEXT: extend these observations and show that the MetR protein also stimulates the in-vitro expression of metH and that both the MetE and MetH proteins synthesized in-vitro are enzymatically active
+  COMP: conjunct[33..58]
+  COMP: conjunction[59..62]
+  COMP: conjunct[63..224]
+SIMP:
+  TYPE: sentence coordination[68..224]
+  TEXT: that the MetR protein also stimulates the in-vitro expression of metH and that both the MetE and MetH proteins synthesized in-vitro are enzymatically active
+  COMP: conjunct[68..137]
+  COMP: conjunction[138..141]
+  COMP: conjunct[142..224]
+

+TYPE: sentence[0..218]
+TEXT: In spite of this , MarA ( E89A ) activation was greater than that of WT MarA for inaA , sodA , and ybjC ; it was comparable to that of WT MarA for micF and less than that of WT MarA for fumC , mdaB , nfsB , and ybhW  .
+POS : In_IN spite_NN of_IN this_DT ,_, MarA_NN -LRB-_-LRB- E89A_NN -RRB-_-RRB- activation_NN was_VBD greater_JJR than_IN that_DT of_IN WT_NNP MarA_NNP for_IN inaA_NNP ,_, sodA_NNP ,_, and_CC ybjC_NN ;_: it_PRP was_VBD comparable_JJ to_TO that_DT of_IN WT_NNP MarA_NNP for_IN micF_NN and_CC less_JJR than_IN that_DT of_IN WT_NNP MarA_NNP for_IN fumC_NNP ,_, mdaB_NN ,_, nfsB_NN ,_, and_CC ybhW_NN ._. 
+TREE: (ROOT (S (S (PP (IN In) (NP (NP (NN spite)) (PP (IN of) (NP (NP (DT this)) (, ,) (NP (NP (NN MarA)) (PRN (-LRB- -LRB-) (NP (NN E89A)) (-RRB- -RRB-))))))) (NP (NN activation)) (VP (VBD was) (ADJP (JJR greater)) (PP (IN than) (NP (NP (DT that)) (PP (IN of) (NP (NP (NNP WT) (NNP MarA)) (PP (IN for) (NP (NP (NNP inaA)) (, ,) (NP (NNP sodA)) (, ,) (CC and) (NP (NN ybjC)))))))))) (: ;) (S (NP (PRP it)) (VP (VBD was) (ADJP (ADJP (JJ comparable) (PP (TO to) (NP (NP (DT that)) (PP (IN of) (NP (NP (NNP WT) (NNP MarA)) (PP (IN for) (NP (NN micF)))))))) (CC and) (ADJP (JJR less))) (PP (IN than) (NP (NP (DT that)) (PP (IN of) (NP (NP (NNP WT) (NNP MarA)) (PP (IN for) (NP (NNP fumC))))))) (, ,) (NP (NN mdaB) (, ,) (NN nfsB) (, ,) (CC and) (NN ybhW)))) (. .)))
+SIMP:
+  TYPE: parenthesis[19..30]
+  TEXT: MarA ( E89A
+  COMP: referred noun phrase[19..23]
+  COMP: parenthesized elements[26..30]
+SIMP:
+  TYPE: apposition[12..32]
+  TEXT: this , MarA ( E89A )
+  COMP: referred noun phrase[12..16]
+  COMP: appositive[19..32]
+SIMP:
+  TYPE: noun or noun phrase coordination[81..103]
+  TEXT: inaA , sodA , and ybjC
+  COMP: conjunct[81..85]
+  COMP: conjunct[88..92]
+  COMP: conjunction[95..98]
+  COMP: conjunct[99..103]
+SIMP:
+  TYPE: noun or noun phrase coordination[193..215]
+  TEXT: mdaB , nfsB , and ybhW
+  COMP: conjunct[193..197]
+  COMP: conjunct[200..204]
+  COMP: conjunction[207..210]
+  COMP: conjunct[211..215]
+SIMP:
+  TYPE: adj or adj phrase coordination[113..160]
+  TEXT: comparable to that of WT MarA for micF and less
+  COMP: conjunct[113..151]
+  COMP: conjunction[152..155]
+  COMP: conjunct[156..160]
+

+TYPE: sentence[0..338]
+TEXT: Although the cocrystal structure of MarA with the marRAB marbox DNA  indicates no interaction between the two molecules at this position  , we considered the possibility that steric hindrance between the marbox DNA and MarA could limit activation by MarA when position 12 is not a T residue ( see the Discussion for a fuller treatment ) .
+POS : Although_IN the_DT cocrystal_JJ structure_NN of_IN MarA_NN with_IN the_DT marRAB_NN marbox_NN DNA_NN indicates_VBZ no_DT interaction_NN between_IN the_DT two_CD molecules_NNS at_IN this_DT position_NN ,_, we_PRP considered_VBD the_DT possibility_NN that_IN steric_JJ hindrance_NN between_IN the_DT marbox_NN DNA_NN and_CC MarA_NN could_MD limit_VB activation_NN by_IN MarA_NN when_WRB position_NN 12_CD is_VBZ not_RB a_DT T_NN residue_NN -LRB-_-LRB- see_VB the_DT Discussion_NN for_IN a_DT fuller_JJR treatment_NN -RRB-_-RRB- ._. 
+TREE: (ROOT (S (SBAR (IN Although) (S (NP (NP (DT the) (JJ cocrystal) (NN structure)) (PP (IN of) (NP (NP (NN MarA)) (PP (IN with) (NP (DT the) (NN marRAB) (NN marbox) (NN DNA)))))) (VP (VBZ indicates) (NP (NP (DT no) (NN interaction)) (PP (IN between) (NP (DT the) (CD two) (NNS molecules)))) (PP (IN at) (NP (DT this) (NN position)))))) (, ,) (NP (PRP we)) (VP (VBD considered) (NP (DT the) (NN possibility)) (SBAR (IN that) (S (NP (NP (JJ steric) (NN hindrance)) (PP (IN between) (NP (DT the) (NN marbox) (NN DNA) (CC and) (NN MarA)))) (VP (MD could) (VP (VB limit) (NP (NN activation)) (PP (IN by) (NP (NN MarA))) (SBAR (WHADVP (WRB when)) (S (NP (NN position) (CD 12)) (VP (VBZ is) (RB not) (NP (NP (DT a) (NN T) (NN residue)) (PRN (-LRB- -LRB-) (S (VP (VB see) (NP (NP (DT the) (NN Discussion)) (PP (IN for) (NP (DT a) (JJR fuller) (NN treatment)))))) (-RRB- -RRB-))))))))))) (. .)))
+