To distinguish between these alternatives , we first analyzed the expression of mgtA , pcgL , mgtC , and pmrC as an indirectly PhoP-regulated gene ( 21 , 43 ) in a phoP : : Tn10 strain , expressing PhoP from the IPTG-regu-lated plasmid pEG9014 .
PhoP binds to the promoter region of mgtC .
The PhoP-acti-vated mig-14 , mgtC , virK , and pagC promoters of Salmonella do not harbor a typical PhoP box in place of the 35 region , as found in archetypal PhoP-regulated promoters such as the mgtA promoter ( 13 ) .
The experimental verification that the PhoP protein binds to promoters in-vivo and Fig. 2C , together with the demonstration that the PhoP box is necessary for mgtC transcription despite data not shown , argues against proposals that atypical PhoP-dependent promoters are regulated by the PhoP protein only indirectly .
The experimental verification that the PhoP protein binds to promoters in-vivo and Fig. 2C , together with the demonstration that the PhoP box is necessary for mgtC transcription despite its unusual orientation , argues against proposals that atypical PhoP-dependent promoters are regulated by the PhoP protein only indirectly .
The experimental verification that the PhoP protein binds to promoters in-vivo and in-vitro , together with the demonstration that the PhoP box is necessary for mgtC transcription despite data not shown , argues against proposals that atypical PhoP-dependent promoters are regulated by the PhoP protein only indirectly .
The experimental verification that the PhoP protein binds to promoters in-vivo and in-vitro , together with the demonstration that the PhoP box is necessary for mgtC transcription despite its unusual orientation , argues against proposals that atypical PhoP-dependent promoters are regulated by the PhoP protein only indirectly .
The experimental verification that the PhoP protein binds to different classes in-vivo and Fig. 2C , together with the demonstration that the PhoP box is necessary for mgtC transcription despite data not shown , argues against proposals that atypical PhoP-dependent promoters are regulated by the PhoP protein only indirectly .
The experimental verification that the PhoP protein binds to different classes in-vivo and Fig. 2C , together with the demonstration that the PhoP box is necessary for mgtC transcription despite its unusual orientation , argues against proposals that atypical PhoP-dependent promoters are regulated by the PhoP protein only indirectly .
The experimental verification that the PhoP protein binds to different classes in-vivo and in-vitro , together with the demonstration that the PhoP box is necessary for mgtC transcription despite data not shown , argues against proposals that atypical PhoP-dependent promoters are regulated by the PhoP protein only indirectly .
The experimental verification that the PhoP protein binds to different classes in-vivo and in-vitro , together with the demonstration that the PhoP box is necessary for mgtC transcription despite its unusual orientation , argues against proposals that atypical PhoP-dependent promoters are regulated by the PhoP protein only indirectly .
The level of PagC protein is lowered in a mgtC null mutant : ( A ) , 1-D SDS-PAGE of outer-membrane proteins isolated from 14028s ( wild-type ) or NM14 ( DmgtC ) grown in low Mg2þ medium ( conditions that activate PhoP-regulated genes ) .
To identify the PhoP-regulated gene ( s ) responsible for motility on 0.3 % agarose low Mg2 + media , we tested the behavior of strains mutated in each of 19 different PhoP-activated genes ( mgtA , mgtB , mgtC , mig-14 , pagC , pagK , pagM , pagN , pagO , pagP , pcgL , pgtE , phoN , pmrD , rstA , slyA , ugtL , virK , and yobG ) .
This is because the PhoP protein , virulence , activates mgtC transcription directly by binding to the mgtC promoter .
This is because the PhoP protein , a major regulator of Salmonella , activates mgtC transcription directly by binding to recruiting RNA polymerase .
This is because the PhoP protein , a major regulator of Salmonella , activates mgtC transcription directly by binding to the mgtC promoter .
This is because the PhoP protein , a major regulator of Salmonella , activates mgtC transcription directly by binding to the mgtC promoter .
To determine whether acetylation affects the activity of PhoP as a transcription factor , several known PhoP-regulated genes including mgtA , mgtC , pmrA and pagC were selected to evaluate the role of Pat in the regulation of PhoP activity .
To determine whether acetylation affects the activity of PhoP as a transcription factor , mgtC were selected to evaluate the role of Pat in the regulation of PhoP activity .
efp enables differential expression of genes that Pro respond to proline-tRNA levels gene because , like mgtC , it is regulated by the PhoP/absence of supporting inforPhoQ two component system at the transcription initia-mation Fig .
efp enables differential expression of genes that Pro respond to proline-tRNA levels gene because , like mgtC , it is regulated by the PhoP/absence of the efp gene -LRB- Fig. 2B .