RESULTS AND DISCUSSIONS Screen for Fur-regulated Promoters and Sequence Analyses of DNA Regions Identified by FURTA Fur-regulated promoters and iron binding proteins carried on a plasmid can be identified by transference into E. coli H1717 ( Stojiljkovic et al. , 1994 ) , which carries fhuF < lacZ ; a Fur-regulated gene fusion sensitive to changes in repressor ( Fur-Fe ) þ2 concentration .
RESULTS AND DISCUSSIONS Screen for Fur-regulated Promoters and Sequence Analyses of DNA Regions Identified by FURTA Fur-regulated promoters and iron binding proteins carried on a plasmid can be identified by transference into E. coli H1717 ( Stojiljkovic et al. , 1994 ) , which carries fhuF < lacZ ; a Fur-regulated gene fusion sensitive to changes in repressor ( Fur-Fe ) þ2 concentration .
To determine whether Fur regulation of tcf was specific to S. Typhi , lacZ reporter plasmid was transformed into S. Typhimurium .
To determine whether Fur regulation of tcf was specific to S. Typhi , lacZ reporter plasmid was transformed into their isogenic fur mutants .
To determine whether Fur regulation of tcf was specific to S. Typhi , lacZ reporter plasmid was transformed into E. coli K-12 .