In addition , at low-osmolarity , RcsB , negatively controls the transcription of flhDC .
In addition , RcsB inhibits flhDC expression .
ecnR in turn results in repression of flhDC transcription in concert with the RcsB protein
RcsB , is a known repressor of flhDC transcription .
To test a putative link between RcsB in repression of flhDC , we performed an unbiased genetic screen using random transposon mutagenesis .
The transposon mutagenesis thus provided further evidence for a functional link between RcsB in repression of flhDC .
To test for the specificity of RflM in promoting RcsB-dependent repression of flhDC , we analyzed the transcript levels of various RcsB targets in the absence or presence of rflM ing to the RcsCDB regulon were regulated by RcsB independently of the presence of RflM .
To test for the specificity of RflM in promoting RcsB-dependent repression of flhDC , we analyzed the transcript levels of various RcsB targets in the absence or presence of rflM ing to csgD were regulated by RcsB independently of the presence of RflM .
To test for the specificity of RflM in promoting RcsB-dependent repression of flhDC , we analyzed the transcript levels of various RcsB targets in the absence or presence of rflM ing to dps were regulated by RcsB independently of the presence of RflM .
To test for the specificity of RflM in promoting RcsB-dependent repression of flhDC , we analyzed the transcript levels of various RcsB targets in the absence or presence of rflM ing to wzzB were regulated by RcsB independently of the presence of RflM .
This demonstrated that RcsB is indispensable for the rflM-dependent repression of flhDC was analyzed using several rcsB mutants .
flhDC is consistent with previous The role of RcsB in repression of flhDC expression
Coordinated repression of flhDC transcription by RcsB .
A. Schematic regulatory model of repression of the flagellar master regulator flhDC by coordinated action of RcsB .
Moreover , the results above indicated that rcsB overexpression bypassed the need for RcsB phosphorylation in repression of flhDC transcription .
expression of flhC-lac _ providing further evidence for a cooperative action of RcsB in repression of flhDC
In summary , our results demonstrate that RflM protein promotes RcsB target specificity for repression of flhDC Discussion Regulation of flagellar synthesis is controlled by the RcsCDB phosphorelay system in both S. enterica serovar Typhimurium .
In summary , our results demonstrate that RflM protein promotes RcsB target specificity for repression of flhDC Discussion Regulation of flagellar synthesis is controlled by the RcsCDB phosphorelay system in both E. coli .
In E. coli , it has been shown that RcsA , enhan-ces RcsB-dependent repression of flhDC .
In E. coli , it has been shown that an auxiliary regulatory protein , enhan-ces RcsB-dependent repression of flhDC .
We dem-onstrate that RflM mediates target specificity of unphosphoryl-ated RcsB for repression of flhDC .
RcsB-mediated repression of flhDC increased significantly in the presence of RflM
Overexpressed RcsB were able to repress flhDC
As mentioned , we found that phosphorylation of RcsB was not essential for repression of flhDC in the presence of RflM , but for RcsB-mediated repression of flhDC in the absence of RflM .
As mentioned , we found that phosphorylation of RcsB was not essential for repression of flhDC in the presence of RflM , but for RcsB-mediated repression of flhDC in the absence of RflM .
These results indicated that RflM directs RcsB to specifically repress flhDC gene transcription independently of external stimuli .
Phosphorylated RcsB would be able to repress flhDC with low affinity by binding to the RcsB box in the flhDC promoter region as a homodimer .
Phosphorylated RcsB would be able to repress flhDC with low affinity by binding to the RcsB box in the flhDC promoter region as a homodimer .
RcsB is known to repress transcription of flhDC in both S. enterica .
RcsB is known to repress transcription of flhDC in both E. coli .