To circumvent this issue and facilitate the examination of YdcRregulated srfN transcription in-vitro , we further introduced a YdcR-expressing plasmid into the ydcR strain , whose expression is under the control of arabinose .
Herein we quantitatively studied the protein expression of a Salmonella mutant lacking ydcR ( ydcR ) within host cells in comparison to that of its parental strain , which provides a powerful means to uncover potential YdcR-regulated proteins under physiological conditions .
Experimental Design and Statistical Rationale -- To uncover potential YdcR-regulated proteins , we performed proteomic analyses of the intracellular ydcR mutant at 18 hpi , whereas the wild-type strain was used as a control .
To uncover YdcR-regulated proteins , we exploited quantitative proteomics to compare intracellular Sal-monella protein expression between the wild-type and the isogenic ydcR strains .
In contrast , the expression level of PipB did not differ significantly for Salmonella strains chromosomally expressing 3 FLAG-tagged PipB in ydcR deletion backgrounds , indicating the specific regulation of SrfN by YdcR .