On the other hand , LeuO has only been found for ompS2 , although there is the distinct possibility that there is such a regulator for ompS1 .
We report here that LeuO are the main negative regulators of ompS1 expression in Salmonella .
We report here that LeuO are the main positive regulators of ompS1 expression in Salmonella .
To test whether LeuO regulated the ompS1 gene , LeuO was expressed in S. Typhi wild type from plasmid pFMTrcleuO-50
Interestingly , the pRO310 ompS1 was also positively regulated by LeuO .
Hence , LeuO regulated positively the ompS1 gene .
LeuO derepressed both ompS1 promoters To elucidate how LeuO regulate ompS1 expression , the effect of LeuO was evaluated in IMSS-1 , in IMSS-41 , in STYhns99 and in the hns ompR double mutant -LRB- STY9941 -RRB- .
LeuO derepressed both ompS1 promoters To elucidate how LeuO regulate ompS1 expression , the effect of LeuO was evaluated in IMSS-1 , in IMSS-41 , in the hns mutant and in the hns ompR double mutant -LRB- STY9941 -RRB- .
LeuO derepressed both ompS1 promoters To elucidate how LeuO regulate ompS1 expression , the effect of LeuO was evaluated in IMSS-1 , in the ompR mutant , in STYhns99 and in the hns ompR double mutant -LRB- STY9941 -RRB- .
LeuO derepressed both ompS1 promoters To elucidate how LeuO regulate ompS1 expression , the effect of LeuO was evaluated in IMSS-1 , in the ompR mutant , in the hns mutant and in the hns ompR double mutant -LRB- STY9941 -RRB- .
LeuO derepressed both ompS1 promoters To elucidate how LeuO regulate ompS1 expression , the effect of LeuO was evaluated in the wild type , in IMSS-41 , in STYhns99 and in the hns ompR double mutant -LRB- STY9941 -RRB- .
LeuO derepressed both ompS1 promoters To elucidate how LeuO regulate ompS1 expression , the effect of LeuO was evaluated in the wild type , in IMSS-41 , in the hns mutant and in the hns ompR double mutant -LRB- STY9941 -RRB- .
LeuO derepressed both ompS1 promoters To elucidate how LeuO regulate ompS1 expression , the effect of LeuO was evaluated in the wild type , in the ompR mutant , in STYhns99 and in the hns ompR double mutant -LRB- STY9941 -RRB- .
LeuO derepressed both ompS1 promoters To elucidate how LeuO regulate ompS1 expression , the effect of LeuO was evaluated in the wild type , in the ompR mutant , in the hns mutant and in the hns ompR double mutant -LRB- STY9941 -RRB- .
that LeuO bound upstream from the ompS1 regulatory region , overlapping with one binding site of StpA , but not interacting with the promoter region and promoting a change in the local DNA architecture
that LeuO bound upstream from the ompS1 regulatory region , overlapping with one binding site of H-NS , but not interacting with the promoter region and promoting a change in the local DNA architecture
The observation that both ompS1 were regulated by LeuO at different levels of induction opens the possibility that there is a regulon in S. enterica .
Bacterial strains IMSS-II ( Salmonella serovar Typhi IMSS-1 harboring plasmid pFMTrc12 ) and IMSS-III ( Salmonella serovar Typhi IMSS-1 harboring plasmid pFMTrcleuO-50 ) were independently transformed with fusions of each of the LeuO-regulated genes ( pKK232-9 [ STY3070 ] , pKK232-9 [ assT ] , pKK232-9 [ ompS1 ] , pKK232-9 [ ompS2 ] , pKK232-9 [ tpx ] , pKK232-9 [ ompX ] , and pKK232-9 [ STY1978 ] ) to generate individual strains containing the LeuO regulator , as well as the promoter region of the LeuO-dependent gene .
LeuO regulates the ompS1 , ompS2 , dsrA , and rovA genes .
LeuO are involved in regulation of ompS1 .
Moreover it has been reported that LeuO are the main negative regulators of ompS1 expression in Salmonella .
Moreover it has been reported that LeuO are the main positive regulators of ompS1 expression in Salmonella .