172 , No. 2 In Vitro Interactions of CysB Protein with the cysJIH Promoter of Salmonella typhimurium : Inhibitory Effects of NICHOLAS M. KREDICH * Departments of Biochemistry , Dluke University Medical Center , Durham , North Carolina 27710 The cysteine regulon of Salmonella typhimurium is positively regulated by the CysB protein .
172 , No. 2 In Vitro Interactions of CysB Protein with the cysJIH Promoter of Salmonella typhimurium : Inhibitory Effects of NICHOLAS M. KREDICH * Departments of Medicine , Dluke University Medical Center , Durham , North Carolina 27710 The cysteine regulon of Salmonella typhimurium is positively regulated by the CysB protein .
172 , No. 2 In Vitro Interactions of CysB Protein with the cysJIH Promoter of Salmonella typhimurium : Inhibitory Effects of Sulfide JACEK OSTROWSKI of Biochemistry , Dluke University Medical Center , Durham , North Carolina 27710 The cysteine regulon of Salmonella typhimurium is positively regulated by the CysB protein .
172 , No. 2 In Vitro Interactions of CysB Protein with the cysJIH Promoter of Salmonella typhimurium : Inhibitory Effects of Sulfide JACEK OSTROWSKI of Medicine , Dluke University Medical Center , Durham , North Carolina 27710 The cysteine regulon of Salmonella typhimurium is positively regulated by the CysB protein .
172 , No. 2 In Vitro Interactions of CysB Protein with the cysJIH Promoter of Salmonella typhimurium : Inhibitory Effects of NICHOLAS M. KREDICH * Departments of Biochemistry , Dluke University Medical Center , Durham , North Carolina 27710 The cysteine regulon of Salmonella typhimurium is positively regulated by an inducer .
172 , No. 2 In Vitro Interactions of CysB Protein with the cysJIH Promoter of Salmonella typhimurium : Inhibitory Effects of NICHOLAS M. KREDICH * Departments of Medicine , Dluke University Medical Center , Durham , North Carolina 27710 The cysteine regulon of Salmonella typhimurium is positively regulated by an inducer .
172 , No. 2 In Vitro Interactions of CysB Protein with the cysJIH Promoter of Salmonella typhimurium : Inhibitory Effects of Sulfide JACEK OSTROWSKI of Biochemistry , Dluke University Medical Center , Durham , North Carolina 27710 The cysteine regulon of Salmonella typhimurium is positively regulated by an inducer .
172 , No. 2 In Vitro Interactions of CysB Protein with the cysJIH Promoter of Salmonella typhimurium : Inhibitory Effects of Sulfide JACEK OSTROWSKI of Medicine , Dluke University Medical Center , Durham , North Carolina 27710 The cysteine regulon of Salmonella typhimurium is positively regulated by an inducer .
L-Cysteine did not affect in-vitro-transcription initiation or binding of CysB protein to the cysJIH promoter region .
CysB protein at concentrations as high as 25 jig/ml did bind a small contaminant of cysJIH promoter fragment .
The gel mobility-shift assay was used to study in-vitro effects of L-cysteine on the binding of CysB protein to cysJIH promoter DNA .
The gel mobility-shift assay was used to study in-vitro effects of sulfide on the binding of CysB protein to cysJIH promoter DNA .
that CysB protein binds to the cysJIH promoter region in-vitro
The ability of sulfide to inhibit binding of CysB protein to the cysJIH promoter is competitive with N-acetyl-L-serine .
The ability of sulfide to inhibit binding of CysB protein to the cysJIH promoter parallels its effects on transcription initiation .
Hryniewicz , M.M. , and Kredich , N.M. Stoichiometry of binding of CysB to the cysJIH , cysK , and cysP promoter regions of Salmonella typhimurium .
Stoichiometry of binding of CysB to the cysJIH .
CysB , in turn , is a positive regulator of cysJIH .
Taken together , our results suggests that CysB regulates expression of cysJIH operon to produce H2S as a cellular protector during oxidative-stress in S. Typhimurium .
cysJIH expression is upregulated by CysB in response to menadione In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysH genes in DcysB strains in sulfate medium .
cysJIH expression is upregulated by CysB in response to menadione In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysH genes in wild type in sulfate medium .
cysJIH expression is upregulated by CysB in response to menadione In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysI genes in DcysB strains in sulfate medium .
cysJIH expression is upregulated by CysB in response to menadione In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysI genes in wild type in sulfate medium .
cysJIH expression is upregulated by CysB in response to menadione In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysJ genes in DcysB strains in sulfate medium .
cysJIH expression is upregulated by CysB in response to menadione In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysJ genes in wild type in sulfate medium .
cysJIH expression is upregulated by CysB in response to ceftriaxone In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysH genes in DcysB strains in sulfate medium .
cysJIH expression is upregulated by CysB in response to ceftriaxone In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysH genes in wild type in sulfate medium .
cysJIH expression is upregulated by CysB in response to ceftriaxone In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysI genes in DcysB strains in sulfate medium .
cysJIH expression is upregulated by CysB in response to ceftriaxone In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysI genes in wild type in sulfate medium .
cysJIH expression is upregulated by CysB in response to ceftriaxone In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysJ genes in DcysB strains in sulfate medium .
cysJIH expression is upregulated by CysB in response to ceftriaxone In order to correlate a possible regulation of cysJIH by CysB in ROS treatment , we evaluated the transcript levels of cysJ genes in wild type in sulfate medium .
In this work , we propose a model and the presence of the transcriptional regulator of CysB , and the products of the cysJIH operon .
olecular characterization of the cysJIH promoters of Salmonella typhimurium : regulation by CysB protein and N-acetyl-L-serine , J. Bacteriol .
M.M. Hryniewicz , N.M. Kredich , Stoichiometry of binding of CysB to the cysJIH , cysK , and cysP promoter regions of Salmonella typhimurium , J. Bacteriol .
Stoichiometry of binding of CysB to the cysJIH .