iroB genes are transcriptionally regulated by Fur
Gene targets included : 16S rDNA ( Lin and Tsen 1996 ) , a Salmonella pathogenicity island I virulence gene ( hilA ) ( Guo et al. 2000 ) , Salmonella enterotoxin gene ( stn ) ( Makino et al. 1999 ) , invA gene ( Ferretti et al. 2001 ) , Fur-regulated gene ( iroB ) ( Bäumler et al. 1997 ) , histidine transport operon ( Cohen et al. 1993 ) , the junction between sipB and sipC virulence genes ( sipB-sipC ) ( Carlson et al. 1999 ) , a Salmon-ella-specific repetitive DNA fragment ( Jitrapakdee et al. 1995 ) , and a multiplex targeting invA gene and spvC gene of the virulence plasmid ( invA/spvC ) ( Chiu and Ou 1996 ) .
To verify if the S. Typhi siderophore salmochelin was regulated by Fur and/or by the sRNAs RfrA and RfrB , a transcriptional-fusion between the iroB promoter , the first gene of the iroBCDE operon , and the lacZ reporter gene was introduced to the different mutant strains .
To verify if the S. Typhi siderophore salmochelin was regulated by Fur and/or by the sRNAs RfrA and RfrB , a transcriptional-fusion between the iroB promoter , the first gene of the iroBCDE operon , and the lacZ reporter gene was constructed in plasmid pRS415 .
that the transcription of iroB is regulated by Fur