Binding of H-NS to the intergenic region caused moderate activation of the regulated csgD promoter .
In the absence of SsrA , unphosphorylated SsrB directs transcription of factors required for biofilm formation specifically by activating csgD ( agfD ) , the master biofilm regulator by disrupting the silenced , H-NS-bound promoter .
Similar binding behavior with D56A SsrB and SsrBc at the H-NS-bound csgD regulatory region was also Figure 5 .
SsrB D56A and SsrBc condense H-NS-bound csgD DNA .
factors _ required for biofilm formation specifically by activating csgD , the master biofilm regulator by disrupting the silenced , H-NS-bound promoter
We further showed that H-NS was a negative regulator of csgD .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to various environmental stimuli due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to various environmental stimuli due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to various environmental stimuli due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to various environmental stimuli due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to osmolality due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to osmolality due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to pH due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to pH due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to temperature due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to temperature due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to starvation due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to starvation due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to osmolality due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to osmolality due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to pH due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to pH due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to temperature due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to temperature due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to starvation due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected H-NS differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to starvation due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
Furthermore , H-NS , was also known to regulate the expression of csgD in S. Typhimurium .
Furthermore , H-NS , was also known to regulate the expression of csgD in E. coli .
SsrB condenses H-NS bound csgD DNA .
It is worth mentioning here that in our AFM images , it was apparent that H-NS was still bound to some regions of the csgD promoter when SsrB condensed the DNA .