Microarray analyses of rcsB-and/or rcsB rcsA-dependent changes in genes expression in S. enterica , E. coli and Erwinia amylovora identified dozens of genes that are directly or indirectly controlled by these regulators ( Wang et al. , 2007 ; 2012 ) To determine which members of the Rcs regulon contribute to persistence within tomatoes , we chose a two-pronged approach : we first defined a set of likely direct RcsAB targets , and then que-ried results of a recently completed high-throughput screen of transposon-tagged Salmonella mutants ( de Moraes et al. , submitted ) to determine which of the RcsAB-regulated genes contribute to the reduced fitness of the mutants .