One such isolate was re-constructed by P22 HT transduction to confirm that the Tn10dCm insertion suppressed hilC : : lac downregulation by LeuO .
One such isolate was purified by P22 HT transduction to confirm that the Tn10dCm insertion suppressed hilC : : lac downregulation by LeuO .
Use of a HilE − null mutant constructed ad ( strain SV55 provided independent evidence that lack of HilE suppressed hilC : : lac downregulation by LeuO .
Use of a HilE − null mutant constructed ad d h provided independent evidence that lack of HilE suppressed hilC : : lac downregulation by LeuO .