Moreover , Fur significantly regulates translational hilD
Given that HilD is required for Fur regulation of hilA , it seemed possible that Fur regulates hilD expression in some manner .
To further examine potential Fur-mediated regulation of hilD , w was dependent on both transcription and translation of hilD .
Again , when hilD was deleted in these strains , Fur regulation of hilD was abrogated , while overproduction of HilC still induced hilD expression .
When the hilD translational fusion was placed under control of the lacUV5 promoter , overproduction of Fur had no effect .
Taken together , these data rule out the possibility that there is Fur control of SPI1 by simple activation of hilD transcription or translation .
Fur regulation of hilD requires the HilD protein .
Fur does not directly regulate hilD transcription .
Although data suggest that hilD is not controlled at the translational level , we wanted to determine if RyhB was involved in Fur-mediated regulation of SPI1 .
Although data suggest that hilD is not controlled at the translational level , we wanted to determine if RyhB was involved in Fur-mediated regulation of SPI1 .
If small RNAs are involved in the Fur regulation of hilD , deletion of hfq could increase expression of hilA in the fur background .
Fur directly binds to the hilD promoter .
Metal-bound Fur has also been shown to bind to an AT-rich region of the hilD promoter to stimulate hilD transcription .