Falkow , S. OmpR regulates the two-component system ssrA ± ssrB in Salmo-nella pathogenicity island 2 .
Lee , A.K. , Detweiler , C.S. , , S. OmpR regulates the two-component system ssrA ± ssrB in Salmo-nella pathogenicity island 2 .
OmpR binds directly to the promoter of ssrA .
Phosphorylation of OmpR has a large effect on DNA binding at ssrA
Phosphorylation of OmpR stimulates DNA binding to ssrA We next measured equilibrium binding of OmpR-P to the ssrA-1 binding site .
Phosphorylation of OmpR stimulates DNA binding to ssrA We next measured equilibrium binding of OmpR to the ssrA-1 binding site .
OmpR did not examine the effect of OmpR phosphorylation on DNA binding at ssrA
that OmpR binds directly to the ssrA to affect their expression
Previous results from gene fusions suggest that regulation of ssrA by OmpR is distinct .
Previous results from gene fusions suggest that regulation of ssrA by OmpR is uncoupled .
Previous results from primer-extension analysis suggest that regulation of ssrA by OmpR is distinct .
Previous results from primer-extension analysis suggest that regulation of ssrA by OmpR is uncoupled .
Other examples of genes include ssrA known regulators of SPI-2 previously reported to be influenced by OmpR Figure 7 .
Other examples of genes include ssrA known regulators of SPI-2 previously reported to be influenced by OmpR Figure 7 .
previous work in Salmonella has shown that phosphorylated OmpR can bind to either sensor kinase gene ssrA within SPI-2
In low-osmolarity conditions , OmpR and/or OmpR-P -LRB- phosphorylayted OmpR present in low levels -RRB- can bind to the upstream regions of ssrA genes .
Under low osmolality , the ssrA genes are transcriptionally activated by the binding of OmpR ~ P to their promoters whose levels are in turn .
Under acidic pH , the ssrA genes are transcriptionally activated by the binding of OmpR ~ P to their promoters whose levels are in turn .
The global regulator OmpR regulates positively SsrAB directly by binding to the ssrA promoter .
The global regulator OmpR regulates positively SsrAB directly by binding to the ssrA promoter .
For example , OmpR promotes SPI-2 expression by directly binding to both the ssrA .