Interestingly , the same region upstream of the pagC translational start has been implicated in binding of the transcriptional regulatory protein SlyA ( J. Gunn and 700 : lacZ pagD : : .
The pagC genes appear to be regulated by both SlyA , by a mechanism .
Consistently , results from different laboratories showed that SlyA regulates a subset of PhoP-dependent genes , including pagC , in Salmonella .
Although the previous result suggested that SlyA could bind to downstream of the transcription start of pagC , the ATTATT direct repeat we identified should represent namely , the SlyA box .
Although the previous result suggested that SlyA could bind to downstream of the transcription start of pagC , the ATTATT direct repeat we identified should represent the SlyA recognition site .
twice that _ controlled by up-1 in wild-type cells , indicating that SlyA also facilitates PhoP binding to the pagC promoter
pagC genes are regulated by a similar mechanism , involving the coordinated actions of SlyA .
Moreover , the pagC gene is positively regulated by SlyA .
Within the virulence coincident genes , we determined that pagC is positively regulated by SlyA .
Direct binding to promoter regions of virulence genes To determine whether SlyA regulates pagC genes in-vivo , the promoter activity of each gene was evaluated using the vector pGLO in DslyA strains under conditions of oxidative-stress .
Direct binding to promoter regions of virulence genes To determine whether SlyA regulates pagC genes in-vivo , the promoter activity of each gene was evaluated using the vector pGLO in WT strains under conditions of oxidative-stress .
Direct binding to promoter regions of metabolism genes To determine whether SlyA regulates pagC genes in-vivo , the promoter activity of each gene was evaluated using the vector pGLO in DslyA strains under conditions of oxidative-stress .
Direct binding to promoter regions of metabolism genes To determine whether SlyA regulates pagC genes in-vivo , the promoter activity of each gene was evaluated using the vector pGLO in WT strains under conditions of oxidative-stress .
SlyA interaction with the promoter region of pagC occurred at a SlyA concentration of Fig. 4G , suggesting that SlyA directly regulates pagC expression .
SlyA interaction with the promoter region of pagC occurred at a SlyA concentration of 9.09 nM , suggesting that SlyA directly regulates pagC expression .
Direct regulation of pagC genes by SlyA in response to ROS .