Even a recent report in which the proteomes of wild type , hilA null ( a transcriptional regulator of SPI-1 genes ) and ssrB null were analyzed by SILAC and compared with an existing CHIP dataset failed to identify csgD as an SsrB-regulated locus ( Brown et al. , 2014 ) , as sequence gazing alone does not help in identifying mechanisms of transcriptional regulation .
AFM imaging revealed that unphosphorylated SsrB was able to bind to the csgD regulatory region
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to various environmental stimuli due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to various environmental stimuli due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to various environmental stimuli due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to various environmental stimuli due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to osmolality due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to osmolality due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to pH due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to pH due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to temperature due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to temperature due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to starvation due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in S. Typhimurium , the expression of csgD is sensitive to starvation due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to osmolality due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to osmolality due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to pH due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to pH due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to temperature due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to temperature due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to starvation due to the action of upstream global regulators at the intergenic region of the csgBAC operons .
CsgD levels were undetected SsrB differentially regulate csgD expression Both in E. coli , the expression of csgD is sensitive to starvation due to the action of upstream global regulators at the intergenic region of the csgDEFG operons .
Unphosphorylated SsrB binds to the csgD regulatory region SsrB is X-ray crystallography suggested that phosphorylation was required for DNA binding .
Unphosphorylated SsrB binds to the csgD regulatory region SsrB is a NarL family member suggested that phosphorylation was required for DNA binding .
Surprisingly , we observed binding of SsrB , D56A SsrB and SsrBc to distinct regions of the csgD regulatory region .
Binding showed that unphosphorylated SsrB was capable of binding csgD .
Closer examination revealed a sharp curvature at the regions where SsrB was bound to csgD .
Thus , unphosphorylated SsrB can bind to the csgD regulatory region when it has been coated with the repressor H-NS .
complexes _ formed by the binding of SsrB and/or H-NS to the csgD regulatory region