HilD binds directly to the regulatory regions of the ssrAB operon .
HilD binds to the regulatory region of ssrAB , promoting OmpR binding .
HilD binds to the regulatory region of ssrAB , thereby counteracting H-NS mediated repression .
Furthermore , electrophoretic-mobility-shift assays showed that both HilD bind to the ssrAB region containing the repressing sequences .
the mechanism by which HilD regulate the expression of ssrAB for the first time that HilD is able to displace H-NS from one of its target genes
RESULTS cis elements required for the regulation of ssrAB by HilD .
all the cis elements _ required for the HilD-mediated regulation of ssrAB
the 302/478 region _ required for the regulation of ssrAB by HilD
negative regulatory sequences are required to maintain the HilD-mediated regulation of ssrAB
the cis elements _ required for the HilD-mediated regulation of ssrAB
The expression analysis of ssrAB-cat 10 fusions in the hilD mutant revealed that consecutive deletions of the 240 / FIG 3 Analysis of the cis-acting sequences required for the HilD-mediated regulation of ssrAB .
The expression analysis of ssrAB-cat 69 revealed that consecutive deletions of the 240 / FIG 3 Analysis of the cis-acting sequences required for the HilD-mediated regulation of ssrAB .
The expression analysis of ssrAB-cat 119 revealed that consecutive deletions of the 240 / FIG 3 Analysis of the cis-acting sequences required for the HilD-mediated regulation of ssrAB .
The expression analysis of ssrAB-cat 240 revealed that consecutive deletions of the 240 / FIG 3 Analysis of the cis-acting sequences required for the HilD-mediated regulation of ssrAB .
The expression analysis of the ssrAB-cat 336 revealed that consecutive deletions of the 240 / FIG 3 Analysis of the cis-acting sequences required for the HilD-mediated regulation of ssrAB .
Our data from the expression analysis showed that HilD regulate the expression of ssrAB mainly by acting on the 119/336 regions , respectively .
Our data from the expression analysis showed that HilD regulate the expression of ssrAB mainly by acting on the 55/240 regions , respectively .
To further dissect ated regulation of ssrAB , we analyzed the interaction of HilD to different segments of the 302/478 region by EMSAs .
To further dissect ated regulation of ssrAB , we analyzed the interaction of HilD to different segments of the 302/478 region by electro-phoretic mobility-shift assays .
FIG 5 HilD bind to the same regions of ssrAB .
DISCUSSION In this study , we elucidated the mechanism by which HilD regulate the expression of ssrAB .
the cis elements _ required for positive HilD-mediated regulation of ssrAB
HilD binds to close to most of the sites of ssrAB .
The regions _ required for the regulation of ssrAB by HilD
To determine how HilD to regulate the expression of ssrAB in response to different growth-conditions is a matter of future studies .
To determine how HilD to regulate the expression of ssrAB in response to different growth-conditions is a matter of our current .
HilD cooperate to directly control the expression of ssrAB in our unpublished results .
HilD cooperate to directly control the expression of ssrAB in SPI-1-inducing conditions .
reciprocally , SPI-1-encoded HilD upregulates SPI-2 gene expression by directly binding the ssrAB operon