Because PhoP/PhoQ regulates more than 40 genes , additional PhoP/PhoQ-activated genes , including pmrD and pagD , were examined to determine if bile specifically regulated pagC , or if it has a general effect on PhoP/PhoQ-regulated genes .
The results establish that other PhoP/PhoQ-regulated genes were unaffected by the presence of bile ( as exemplified by pagD and pmrD , Fig. 2 ) , suggesting that pagC is specifically affected by bile .