Furthermore , preliminary results indicate that HilD can bind directly to the invF promoter , supporting the hypothesis that there is an alternative HilA-independent mechanism for C. P. Lostroh , unpublished results .
Furthermore , preliminary results indicate that HilD can bind directly to the invF promoter , supporting the hypothesis that there is an alternative HilA-independent mechanism for activating invF .
We show that HilD can directly bind invF .
Overproduction of HilD activate the expression of a chromosomal invF-lacZ fusion in the absence of hilA In order to study the activation of invF by HilD , we examined the expression of a chromosomal invF-lacZ fusion in a hilA hilD mutant strain , which express HilA , respectively , under the control of the arabinose-inducible PBAD promoter .
Overproduction of HilD activate the expression of a chromosomal invF-lacZ fusion in the absence of hilA In order to study the activation of invF by HilD , we examined the expression of a chromosomal invF-lacZ fusion in a hilA hilD mutant strain , which express HilC , respectively , under the control of the arabinose-inducible PBAD promoter .
Overproduction of HilD activate the expression of a chromosomal invF-lacZ fusion in the absence of hilA In order to study the activation of invF by HilD , we examined the expression of a chromosomal invF-lacZ fusion in a hilA hilD mutant strain , which express HilD , respectively , under the control of the arabinose-inducible PBAD promoter .
These results suggest that HilD might directly activate invF expression by binding to sequences upstream of invF .
HilD bind to the invF promoter fragment in-vitro To examine whether HilD might directly activate the invF promoter by binding to sequences upstream of invF , we performed gel shift analyses .
HilD bind to the invF promoter fragment in-vitro To examine whether HilD might directly activate the invF promoter by binding to sequences upstream of invF , we performed gel shift analyses .
In addition to binding upstream of invF , HilD also bind to sites upstream of hilC .
In addition to binding upstream of invF , HilD also bind within S. Akbar , unpublished results .
In addition to binding upstream of invF , HilD also bind within the prgH-hilD intergenic region .
These gel shift assays indicate that HilD bind sequences downstream of the HilD-and HilC-dependent invF promoter .
These gel shift assays indicate that HilD bind sequences upstream of the HilD-and HilC-dependent invF promoter .
given the cross regulation of SPI-2 by the SPI-1-encoded HilD regulator , it is possible that in-vivo invF is needed to control other genes in Salmonella
given the cross regulation of SPI-1 by the SPI-1-encoded HilD regulator , it is possible that in-vivo invF is needed to control other genes in Salmonella
HilD also directly controls the expression of invF .
However , HilD regulates invF by binding to downstream of the HilC/Ddependent invF promoter .
However , HilD regulates invF by binding to downstream of the HilC/Ddependent invF promoter .
However , HilD regulates invF by binding to downstream of the HilC/Ddependent invF promoter .
However , HilD regulates invF by binding to regions upstream .
However , HilD regulates invF by binding to regions located .
invF is positively regulated by HilD through HilA