Expression of assT was induced in an hns mutant , consistent with the notion that H-NS represses these genes ; transcriptional activity was lower for STY1978 in an hns background , suggesting that this global regulatory protein has a positive effect .
The transcriptional profile was consistent with the hypothesis that H-NS represses the expression of assT , .
The results of our experiments concur with the concept that H-NS represses the expression of assT in Salmonella serovar Typhi .
Previous studies also showed that assT are repressed by H-NS in Salmo-nella serovar Typhimurium .
The H-NS nucleoid protein repressed the expression of the assT transcriptional units .
Since previous reports have shown that H-NS negatively regulates assT transcription in S. Typhi IMSS-1 , the assT transcriptional-fusions were analyzed in an hns background , in N-minimal-medium .
In contrast , low levels of assT expression were detected in the absence of P2 , in a STYhns99 strain , suggesting that the upstream PLeuO promoter activity is also repressed by the H-NS global regulator in N-minimal-medium .
In contrast , low levels of assT expression were detected in the absence of P1 , in a STYhns99 strain , suggesting that the upstream PLeuO promoter activity is also repressed by the H-NS global regulator in N-minimal-medium .