Although full resist-ance to polymyxin B requires pmrA , these genes appear to be differentially regulated by PhoP in that transcription of Fig. 1A
Although full resist-ance to polymyxin B requires pmrA , these genes appear to be differentially regulated by PhoP in that transcription of the former
The ugtL gene mediates polymyxin B resistance independently of the PmrA-PmrB system phoP Salmonella are > 20 times more polymyxin sensitive than a pmrA mutant when bacteria are grown in low ygjU 3394555 3392617 rfaB rfaI 3914096 3915562 slsA STM3760 cigR 3959077 3960805 rhaT rhaR 4260472 4262386 STM0725 STM0726 STM0724 792487 790384 Mg2 + ( Wosten et al. , 2000 ) , indicating that a PmrA-inde-pendent PhoP-regulated gene ( s ) is necessary for poly-myxin resistance .
The ugtL and pmrA genes mediate PhoP-controlled resistance to polymyxin B. A. Percentage survival of wild-type ( 14028s ) , phoP ( MS7953s ) and ugtL ( EG13682 ) strains after incubation with polymyxin B ( 1.0 mg ml-1 ) .
A phoP mutant is > 20 times more polymyxin B sensitive than a pmrA mutant when bacteria are grown in low Mg2 + and > 20 times more sensitive than wild-type Salmonella when bacteria are grown in the presence of Fe3 + ( Wosten et al. , 2000 ) , indicating that a PhoP-regulated PmrA-independent gene ( s ) participate ( s ) in polymyxin B resistance .
To determine whether acetylation affects the activity of PhoP as a transcription factor , several known PhoP-regulated genes including mgtA , mgtC , pmrA and pagC were selected to evaluate the role of Pat in the regulation of PhoP activity .
To determine whether acetylation affects the activity of PhoP as a transcription factor , pmrA were selected to evaluate the role of Pat in the regulation of PhoP activity .