We show that HilC can directly bind invF .
Overproduction of HilC activate the expression of a chromosomal invF-lacZ fusion in the absence of hilA In order to study the activation of invF by HilC , we examined the expression of a chromosomal invF-lacZ fusion in a hilA hilD mutant strain , which express HilA , respectively , under the control of the arabinose-inducible PBAD promoter .
Overproduction of HilC activate the expression of a chromosomal invF-lacZ fusion in the absence of hilA In order to study the activation of invF by HilC , we examined the expression of a chromosomal invF-lacZ fusion in a hilA hilD mutant strain , which express HilC , respectively , under the control of the arabinose-inducible PBAD promoter .
Overproduction of HilC activate the expression of a chromosomal invF-lacZ fusion in the absence of hilA In order to study the activation of invF by HilC , we examined the expression of a chromosomal invF-lacZ fusion in a hilA hilD mutant strain , which express HilD , respectively , under the control of the arabinose-inducible PBAD promoter .
These results suggest that HilC might directly activate invF expression by binding to sequences upstream of invF .
HilC bind to the invF promoter fragment in-vitro To examine whether HilC might directly activate the invF promoter by binding to sequences upstream of invF , we performed gel shift analyses .
HilC bind to the invF promoter fragment in-vitro To examine whether HilC might directly activate the invF promoter by binding to sequences upstream of invF , we performed gel shift analyses .
In addition to binding upstream of invF , HilC also bind to sites upstream of hilC .
In addition to binding upstream of invF , HilC also bind within S. Akbar , unpublished results .
In addition to binding upstream of invF , HilC also bind within the prgH-hilD intergenic region .
These gel shift assays indicate that HilC each bind sequences downstream of the HilD-and HilC-dependent invF promoter .
These gel shift assays indicate that HilC each bind sequences upstream of the HilD-and HilC-dependent invF promoter .
Dam-dependent regulation of invF was still observed in HilC