Nevertheless , this effect was abolished in the rpoS mutant , indicating that induction of hlyE transcription is dependent on RpoS under these conditions .
These results show that hlyE transcription is increased by low pH and high-osmolarity in an RpoS-dependent manner .
hlyE gene transcription is induced by low pH in an RpoS-dependent manner .
hlyE gene transcription is induced by high-osmolarity in an RpoSdependent manner .
Discussion In this work , we showed that hlyE genes found in SPI-18 present RpoS-dependent induction at low pH or high-osmolarity .
In this work , we showed that the hlyE is upregulated in low pH and high-osmolarity in an RpoSdependent manner .
Finally , rpoS is epistatic over phoPQ , reinforcing the fact that the induction of the hlyE expression under low concentration of Mg2 + occurs via RpoS .
In this paper we showed that RpoS is the most import-ant activator of hlyE expression .
Furthermore , hlyE induction by high-osmolarity is RpoS dependent .