Iron depletion also eliminated the regulatory effect of RstA on the Fur-repressed genes : in bacterial cells grown in LB-medium containing the iron-specific chelator dipyridyl , the transcription levels of the fhuA and fhuF genes were increased to the levels observed in the fur deletion strain even when the RstA protein was overexpressed ( Fig. 1B ) .
We next compared the mRNA levels of fhuF between the feoA promoter mutant strains found that the lack of RstA-activated feoAB transcription abrogates repression of the Fur target gene even in the strain .
We next compared the mRNA levels of fhuF between the wild-type promoter mutant strains found that the lack of RstA-activated feoAB transcription abrogates repression of the Fur target gene even in the strain .