Lee , H.J. , Park , S.J. , Choi , S.H. , Lee , K.H. Vibrio vulnificus rpoS expression is repressed by direct binding of cAMP-CRP complex to its two promoter regions .
The binding of cAMP-CRP complex to rpoS promoter were proved through exogenous cAMP addition , respectively .
The binding of cAMP-CRP complex to rpoS promoter were proved through lacZ fusion , respectively .
The binding of cAMP-CRP complex to rpoS promoter were proved through electrophoretic-mobility-shift assay , respectively .
The whole rpoS promoter bound to CRP in data not shown .
The whole rpoS promoter bound to CRP in EMSA .
The cAMP-CRP complex is thought to be involved in the control of rpoS transcription
cAMP-CRP specifically bound to two sites of the rpoS promoter in-vitro .
It suggests that , except for cAMP-CRP , additional factors may also be involved in the regulation of rpoS transcription .
On the other hand , it has been reported that rpoS expression is repressed by direct binding of CRP-cAMP to its promoter region in Vibrio vulnificus .
Sun demonstrated direct binding of CRP to the rpoS promoter region in S. Typhimurium , where it is able to bind to two sites in this sequence .
Cheng demonstrated direct binding of CRP to the rpoS promoter region in S. Typhimurium , where it is able to bind to two sites in this sequence .
Both sites are conserved in S. Typhi rpoS promoter , suggesting a dir-ect binding by CRP .
This versatility can be explained , at least in part , by the fact that CRP is regulating rpoS .