As suggested by our previous study , establishing the Mg2-responsive regulation of these loci requires the transcriptional repressor H-NS ; thus the mRNA level is reduced greatly in the slyA mutants in low-Mg2 conditions .
As suggested by our previous study , establishing the Mg2-responsive regulation of these loci requires the transcriptional repressor H-NS ; thus the mRNA level is reduced greatly in the slyA mutants in PhoP-activating conditions .
Thus , one explanation for the transcription we observe following overexpression of slyA may be that both SlyA and SsrB counteract binding of both H-NS and YdgD/Hha in this A+T rich SPI-2 region .