A ugtL pagP double mutant was more sensitive than either the ugtL or pagP single mutants but still more resistant than the phoP mutant ( Fig. 2C ) , indicating that the ugtL and pagP genes participate in different pathways of magainin 2 resistance and that additional PhoP-regulated genes contribute to magainin 2 resistance .
In addition to ugtL , PhoP-regulated magainin 2 resistance requires the pagP and yqjA genes ( Fig. 2C ) .
The PhoP-regulated genes described and the modifications their products mediate are : pagP , palmitoyl transferase ; lpxO , formation of 2-hydroxy myristate ; pagL , 3-O-deacylase .
To identify the PhoP-regulated gene ( s ) responsible for motility on 0.3 % agarose low Mg2 + media , we tested the behavior of strains mutated in each of 19 different PhoP-activated genes ( mgtA , mgtB , mgtC , mig-14 , pagC , pagK , pagM , pagN , pagO , pagP , pcgL , pgtE , phoN , pmrD , rstA , slyA , ugtL , virK , and yobG ) .
The overlap in genes either downregulated or upregulated in bile between all strains was small ; only one gene ( pagP ) , a PhoP-PhoQ-regulated gene involved in modifying lipid-A ( 24 ) , was downregulated in all strains ( Fig. 2 ) .