Lee , H.J. , Park , S.J. , Choi , S.H. , Lee , K.H. Vibrio vulnificus rpoS expression is repressed by direct binding of cAMP-CRP complex to its two promoter regions .
In E. coli , CRP-cAMP represses rpoS transcription during logarithmic growth .
CRP participate in the repression of hlyE transcription by repressing rpoS transcription To find out whether CRP is repressing hlyE expression through a direct binding to the respective promoter region , we performed a shift mobility assay .
Previously , it has been reported that rpoS expression is repressed by the cAMP-CRP complex in Vibrio vulnificus .
Thus , CRP might repress hlyE expression by repressing rpoS in S. Typhi .
Altogether , these results suggest that CRP are able to repress the rpoS expression by direct binding , thereby regulating hlyE expression in S. Typhi .
Figure 2 CRP participate in the repression of rpoS .
Here , we present genetic evidence indicating that CRP exerts down-regulation of hlyE by repressing rpoS in S. Typhi .
On the other hand , it has been reported that rpoS expression is repressed by direct binding of CRP-cAMP to its promoter region in Vibrio vulnificus .