Furthermore , electrophoretic-mobility-shift assays showed that both H-NS bind to the ssrAB region containing the repressing sequences .
the mechanism by which H-NS regulate the expression of ssrAB for the first time that HilD is able to displace H-NS from one of its target genes
Our data from the expression analysis showed that H-NS regulate the expression of ssrAB mainly by acting on the 119/336 regions , respectively .
Our data from the expression analysis showed that H-NS regulate the expression of ssrAB mainly by acting on the 55/240 regions , respectively .
To further dissect ated regulation of ssrAB , we analyzed the interaction of H-NS to different segments of the 302/478 region by EMSAs .
To further dissect ated regulation of ssrAB , we analyzed the interaction of H-NS to different segments of the 302/478 region by electro-phoretic mobility-shift assays .
H-NS bind to the same regions of ssrAB .
DISCUSSION In this study , we elucidated the mechanism by which H-NS regulate the expression of ssrAB .
Furthermore , we showed that H-NS binds to the region 111 to 287 of ssrAB .
Furthermore , our EMSAs revealed that H-NS binds to at least two different sites along the 111/287 region of ssrAB .
the model in which H-NS represses the expression of ssrAB initially by binding by forming a nucleoprotein filament on the promoter
The regions _ required for the regulation of ssrAB by H-NS