HilC do not seem to regulate expression of hilD .
These results indicate that Lon is involved in the autoregulation of hilD transcription by modulating amounts of HilC .
Thus , HilC are also apparently indirectly regulating hilD , contributing to the feedforward loop .
This work demonstrates the regulation of hilD by HilC .
When the hilD translational fusion was placed under control of the lacUV5 promoter , overproduction of HilC had no effect .
Thus , although HilD is capable of auto-regulation , the results could not be due to the loss of this regulatory protein controlling its own expres ¬ Pat post-transcriptionally regulates HilD The regulation of hilD is complex ; hilD is also regulated by the transcriptional regulators HilC and RtsA , which constitute a feed-forward circuit to control the downstream SPI1 regulator Hil .