% surviva A B C 100 10 1 r r to to gtL c c u ve ve p e L tL + + + typ gt ug u-ild w pe P y o-t h ld p w i L e oP rA gtL h m u p p A r m p gt u yp-t ld w i A Mg2 + L L L H b-galactosidase activity YqjA-FLAG w ild-type 14028s p h o yqjA-flag P B yqjA w 10µM Mg2 + 2 + - flag ; phoP 10mM Mg ild-type 5 0 40 p Discussion The PhoP -- PhoQ regulatory system controls resistance to a variety of structurally different antimicrobial peptides ( Groisman et al. , 1992 ) , but the identity of the PhoP-regulated determinants mediating peptide resistance has remained largely unknown .
In addition to ugtL , PhoP-regulated magainin 2 resistance requires the pagP and yqjA genes ( Fig. 2C ) .
the yqjA gene we show is PhoP regulated for resistance to polymyxin B or defensin HNP-1
the yqjA gene we show is PhoP regulated for resistance to magainin 2 HNP-1
the yqjA gene we show is PhoP regulated for resistance to polymyxin B or defensin HNP-1
the yqjA gene we show is PhoP regulated for resistance to magainin 2 HNP-1
This raised the possibility that the yqjA and/or yqjB genes might be regulated by the PhoP protein .
two genes -- yqjA -- are both transcriptionally regulated by the PhoP
Expression of the yqjA gene is controlled by the PhoP protein at the transcriptional level .