Positive regulation by CsgD is indicated by solid lines because putative CsgD binding sites have been found upstream from the csgB promoters .
Indeed , it has been suggested , although it has never been shown , that CsgD binds to conserved 11-bp sequences upstream of the csgB promoters , activating transcription of these genes .
In vitro , unphosphorylated CsgD binds directly to the promoter region of csgB .
To quantitate the degree of CsgD complementation in each strain , we measured the expression of csgB , coding for curli fimbria production , coding for a regulator of cellulose production , using promoter-lux operon fusion plasmids .
To further corroborate the CsgD involvement in the cAMP-mediated control of the expression of biofilm , the csgB expression was monitored in absence of CsgD in both cya genetic backgrounds .
To further corroborate the CsgD involvement in the cAMP-mediated control of the expression of biofilm , the csgB expression was monitored in absence of CsgD in both wt genetic backgrounds .
To further corroborate the CsgD involvement in the cAMP-mediated control of the expression of biofilm , the csgB expression was monitored in presence of CsgD in both cya genetic backgrounds .
To further corroborate the CsgD involvement in the cAMP-mediated control of the expression of biofilm , the csgB expression was monitored in presence of CsgD in both wt genetic backgrounds .