Results Activation of leuO transcription represses SPI-1 To confirm previous evidence indicating that SPI-1 might be repressed by LeuO , we compared the expression of selected SPI-1 genes in the absence of LeuO .
Results Activation of leuO transcription represses SPI-1 To confirm previous evidence indicating that SPI-1 might be repressed by LeuO , we compared the expression of selected SPI-1 genes in the presence .
Single cell analysis of gene expression by flow cytometry confirmed that HilE plays a role in LeuO-mediated repression of SPI-1 : activation of leuO expression decreased the activity of a sipB : : GFP fusion : GFP expression ; a hilE null mutation increased sipB : : GFP expression and reduced the size of OFF subpopulation , in agreement with the role of HilE as a SPI-1 repressor ; activation of leuO expression in a HilE − background yielded a small subpopulation of sipB -
Single cell analysis of gene expression by flow cytometry confirmed that HilE plays a role in LeuO-mediated repression of SPI-1 : activation of leuO expression decreased the activity of a sipB : : GFP fusion : GFP expression ; a hilE null mutation increased sipB : : GFP expression and reduced the size of the SPI-1 subpopulation , in agreement with the role of HilE as a SPI-1 repressor ; activation of leuO expression in a HilE − background yielded a small subpopulation of sipB -
In the absence of HilE , activation of leuO transcription reduced epithelial cell invasion Fig. 7B , thereby providing further evidence for HilE-independent downregulation of SPI-1 by LeuO .
In the absence of HilE , activation of leuO transcription reduced epithelial cell invasion three-to fourfold , thereby providing further evidence for HilE-independent downregulation of SPI-1 by LeuO .