Direct binding to promoter regions of virulence genes To determine whether SlyA regulates sopD , sopE2 , hilA fruk , glpA , kgtP in-vivo , the promoter activity of each gene was evaluated using the vector pGLO in DslyA strains under conditions of oxidative-stress .
Direct binding to promoter regions of virulence genes To determine whether SlyA regulates sopD , sopE2 , hilA fruk , glpA , kgtP in-vivo , the promoter activity of each gene was evaluated using the vector pGLO in WT strains under conditions of oxidative-stress .
Direct binding to promoter regions of metabolism genes To determine whether SlyA regulates sopD , sopE2 , hilA fruk , glpA , kgtP in-vivo , the promoter activity of each gene was evaluated using the vector pGLO in DslyA strains under conditions of oxidative-stress .
Direct binding to promoter regions of metabolism genes To determine whether SlyA regulates sopD , sopE2 , hilA fruk , glpA , kgtP in-vivo , the promoter activity of each gene was evaluated using the vector pGLO in WT strains under conditions of oxidative-stress .
Direct regulation of sopD genes by SlyA in response to ROS .
Our results showed that at 3 h post-infection only , sopD were negatively regulated by SlyA , with DslyA expression increased two-and five-times , respectively .