Activation of feoB transcription by the RstA protein is necessary for repression of iron-responsive genes .
To investigate further whether the Fe transporter FeoB is necessary for repression of iron-responsive genes by the RstA protein , we constructed a strain with a deletion of the feoB gene .
The feoB deletion prevented the RstA-promoted repression of transcription levels of Fig. 3B .
Together with the finding that induction of the feoB gene was necessary for repression of the iron response by the RstA protein ( Fig. 3B ) , our results suggest that iron signaling via the RstA-induced FeoB promotes Fur activity beyond the levels .