the transcriptional results showed that LeuO positively induced assT at different points of the growth curve
A LeuO-dependent promoter is necessary for induction of the complete assT-dsbL-dsbI operon in rich-medium , whereas transcriptional start sites were determined for the dsbL-dsbI transcriptional units in the absence of cloned leuO SPI-2 .
A LeuO-dependent promoter is necessary for induction of the complete assT-dsbL-dsbI operon in rich-medium , whereas transcriptional start sites were determined for assT transcriptional units in the absence of cloned leuO SPI-2 .
A LeuO-dependent promoter is necessary for induction of the complete assT-dsbL-dsbI operon in rich-medium , whereas transcriptional start sites were determined for the dsbL-dsbI transcriptional units in a leuO background , in N-minimal-medium SPI-2 .
A LeuO-dependent promoter is necessary for induction of the complete assT-dsbL-dsbI operon in rich-medium , whereas transcriptional start sites were determined for assT transcriptional units in a leuO background , in N-minimal-medium SPI-2 .
Because LeuO still activates assT , although at 25 % of the wild-type levels , we analyzed , by footprinting of a DNA fragment lacking II sites , whether there are other LeuO-binding sites in this region .
Because LeuO still activates assT , although at 25 % of the wild-type levels , we analyzed , by footprinting of a DNA fragment lacking both LBS I sites , whether there are other LeuO-binding sites in this region .
The data indicate that assT is induced in N-minimal-medium in the absence of LeuO , since the same activity was observed in all of the constructions in a leuO isogenic background -LRB- data not shown -RRB- .
In a subsequent study to pursue more targets in Salmonella Typhi , LeuO was found to also positively regulate assT .