PhoP binds to the promoter region of phoN .
in contrast to what we had observed in the regulation of phoN , activation of pagN was similar in 4550 L1-PQ24 , suggesting that different threshold levels of the activated PhoP are necessary to turn on the expression of different pag genes .
in contrast to what we had observed in the regulation of phoN , activation of pagN was similar in 4550 L1-SDQ24 , suggesting that different threshold levels of the activated PhoP are necessary to turn on the expression of different pag genes .
Interestingly we had observed in the regulation of phoN , activation of pagN was similar in 4550 L1-PQ24 , suggesting that different threshold levels of the activated PhoP are necessary to turn on the expression of different pag genes .
Interestingly we had observed in the regulation of phoN , activation of pagN was similar in 4550 L1-SDQ24 , suggesting that different threshold levels of the activated PhoP are necessary to turn on the expression of different pag genes .
A S. typhimurium strain expressing a reporter fusion to the PhoP-controlled gene encoding acid phosphatase ( phoN ) was used as a wild type ST-PhoQ reporter strain background .
The results for 10 mM and 10 μM Mg2 + were also confirmed by RT-PCR for phoN and four additional PhoP-controlled genes ( data not shown ) , so this phenotype does not appear to be specific to the reporter system used .
phoN are known to be regulated by PhoP ,56
Considering that some PhoP-regulated genes such as pag loci and phoN , which encodes a nonspecific acid phosphatase , are maximally induced under starvation conditions ( Behlau and Miller , 1995 ; Kier et al. , 1997 ) , it is reasonable to conclude that induced synthesis of the tdc genes inside macrophages affects the expression of a subset of PhoP-dependent genes such as SPI2 .
As seen in Fig. 7B , PhoP was able to bind to the phoN promoter .
To identify the PhoP-regulated gene ( s ) responsible for motility on 0.3 % agarose low Mg2 + media , we tested the behavior of strains mutated in each of 19 different PhoP-activated genes ( mgtA , mgtB , mgtC , mig-14 , pagC , pagK , pagM , pagN , pagO , pagP , pcgL , pgtE , phoN , pmrD , rstA , slyA , ugtL , virK , and yobG ) .