If the effect of Cra were direct , Cra would bind to more nonconsensus sites within the iscR region .
If the effect of Cra were direct , Cra would bind to one nonconsensus sites within the iscR region .
To determine whether Cra could bind to the iscR promoter region , EMSAs were performed with purified Cra protein .
Indeed , Cra bound to the iscR promoter region , albeit with relatively low affinity .
The weak binding of Cra to the iscR promoter was not surprising given the lack of a consensus sequence .
Cra binds to the iscR promoter region in-vitro .