LeuO positively regulated the ompS1 expression by derepressing both promoters and antagonizing the negative effect of StpA , displacing them from the 5 ′ regulatory region .
LeuO positively regulated the ompS1 expression by derepressing both promoters and antagonizing the negative effect of H-NS , displacing them from the 5 ′ regulatory region .
Interestingly , the pRO310 ompS1 was also positively regulated by LeuO .
The highest level of ompS1 expression was obtained when LeuO was induced at 100 mM IPTG .
LeuO acted as a derepressor , displacing H-NS from the ompS1 regulatory region The highest level of ompS1 expression was attained either upon induction of LeuO in the wild type or else in an hns stpA background .
Thus , whereas the induction of ompS2 expression by LeuO is from a sole OmpR-dependent promoter , the induction of ompS1 can occur independent of OmpR .
the transcriptional results showed that LeuO positively induced ompS1 at different points of the growth curve
Fernandez-Mora M , Calva E LeuO positively regulate the Salmonella enterica serovar Typhi Flores-Valdez MA , Calva E Negative osmoregulation of the Salmonella ompS1 porin independently of OmpR in an hns background .
Fernandez-Mora M , Puente JL LeuO positively regulate the Salmonella enterica serovar Typhi Flores-Valdez MA , Puente JL Negative osmoregulation of the Salmonella ompS1 porin independently of OmpR in an hns background .
LeuO positively regulates the ompS1 expression by derepressing both the promoters .
LeuO positively regulates the ompS1 expression by antagonizing the negative effect of StpA .
LeuO positively regulates the ompS1 expression by antagonizing the negative effect of H-NS .
Previously , we demonstrated that a TATgTcATAT region located at positions 125 to 134 with respect to the transcriptional start site was necessary for the LeuO induction of the ompS1 gene .
LeuO is known to induce ompS2 expression at a lower concentration than required for the induction of ompS1 , consistent with LeuO .
LeuO is known to induce ompS2 expression at a lower concentration than required for the induction of ompS1 , consistent with LeuO .
In Salmonella enterica serovar Typhi , LeuO activates the expression of ompS1 .