CsrA binds the 50-UTR of the fimAICDHF transcript To further investigate the mechanism , we determined whether CsrA can bind to the 50-UTR of the fimAICDHF transcript by an EMSA .
CsrA binds the 50-UTR of the fimAICDHF transcript To further investigate the mechanism , we determined whether CsrA can bind to the 50-UTR of the fimAICDHF transcript by an EMSA .
These results suggested that CsrA specifically binds the 50-UTR of the fimAICDHF transcript .
These data suggested that CsrA bound specifically to GGA binding sites in the 50-UTR of the fimAICDHF transcript .
Our finding that CsrA binds the 50-UTR of the fimAICDHF transcript raised the possibility that the fimAICDHF genes antagonized the regulatory effects of CsrA by a similar mechanism .
Similarly , binding of CsrA to the 50-UTR of the fimAICDHF transcript had little effect on expression of FimA .
little effect on expression of FimA _ suggesting that the main function 0 for the binding of CsrA to the 5-UTR of the fimAICDHF transcript was not to regulate expression of the
Instead , our data raised the intriguing possibility that binding of the fimAICDHF mRNA to CsrA functioned mainly in regulating CsrA activity .
In contrast , CsrA binding of mRNAs will be affected to a lesser extent by fluctuations in fimAICDHF-transcript levels .
Thus , differences in the affi-nities of different mRNA targets for CsrA are predicted to determine the magnitude by which expression of each target is regulated by the 50-UTR of the fimAICDHF transcript .