HilD , regulates the expression of phoH .
that HilD directly controls the expression of the phoH genes
HilD binds to the regulatory regions of phoH .
To further define whether the HilD-mediated regulation of phoH is indirect , we analyzed the interaction of HilD with the regulatory region of these genes .
To further define whether the HilD-mediated regulation of phoH is direct , we analyzed the interaction of HilD with the regulatory region of these genes .
Together with the expression analyses , these binding assays demonstrate that HilD directly regulates the expression of the phoH genes .
In this work , by applying a clustering method to S. Typhimurium SL1344 global expression data from the COLOMBOS database , we show that most of the known genes regulated by HilD , including the flagellar/chemot-axis genes , are indeed co-expressed with SPI-1 ; moreover , nine novel genes were identified : phoH .
Additionally , we show that HilD can induce the expression of SL4247-cat ; consistently , HilD bound to the regulatory regions of phoH .
Additionally , we show that HilD can induce the expression of SL3812-cat ; consistently , HilD bound to the regulatory regions of phoH .
Additionally , we show that HilD can induce the expression of lpxR-cat ; consistently , HilD bound to the regulatory regions of phoH .
Additionally , we show that HilD can induce the expression of sinR-cat ; consistently , HilD bound to the regulatory regions of phoH .
Additionally , we show that HilD can induce the expression of phoH-cat ; consistently , HilD bound to the regulatory regions of phoH .
Additionally , we show that HilD can induce the expression of gtgE-cat ; consistently , HilD bound to the regulatory regions of phoH .
Additionally , we show that HilD can induce SL1896-cat , transcriptional-fusions , in the E. coli MC4100 strain ; consistently , HilD bound to the regulatory regions of phoH .
Additionally , we show that HilD can induce SL1896-cat , thus in the absence of FlhDC ; consistently , HilD bound to the regulatory regions of phoH .
Additionally , we show that HilD can induce SL1896-cat , thus in the absence of other Salmonella-specific regulators ; consistently , HilD bound to the regulatory regions of phoH .
Thus , HilD directly regulates the expression of the phoH genes , and positively .
HilD binds to the regulatory region of phoH .