that EutR influenced expression of ssrB targets
S8 Fig _ indicating that EutR may regulate SPI-2 expression by binding the ssrB promoter
Fig 4B _ indicating that EutR may regulate SPI-2 expression by binding the ssrB promoter
EMSAs indicated that EutR directly binds the ssrB promoter to activate expression of Fig 4C .
EMSAs indicated that EutR directly binds the ssrB promoter to activate expression of SPI-2 .
Electrophoretic mobility-shift assays indicated that EutR directly binds the ssrB promoter to activate expression of Fig 4C .
Electrophoretic mobility-shift assays indicated that EutR directly binds the ssrB promoter to activate expression of SPI-2 .
To test our findings within the complexities of the in-vivo environment , we assessed EutR-dependent regulation of ssrB using single strain infections .
In vitro assays revealed that EutR directly binds the ssrB promoter to activate SPI-2 expression .