Chromosomally encoded adrA is not required for curli fimbriae in S. Typhimurium UMR1 Overexpression of AdrA from plasmid pWJB30 enhanced the expression of CsgD .
Chromosomally encoded adrA is not required for the expression of CsgD from plasmid pWJB30 enhanced the expression of CsgD .
the regulatory network where CsgD activates transcription of adrA
CsgD also stimulates cellulose production via activation of transcription of adrA .
Experiments with purified CsgD are needed to determine whether CsgD can activate transcription by E S and/or E 70 at the adrA promoters .
In agreement with in-vivo findings ( Romling et al. , 2000 ) , CsgD-activated transcription at the adrA promoter occurred with RNA polymerase loaded with RpoS and RpoD .
However , we discovered that c-di-GMP , an integral part of the regulatory network promoting CsgD expression in S. Typhimurium , is not required for binding of CsgD to the csgBA and adrA promoters or for CsgD-activated transcription .
In addition , CsgD contributes indirectly to cellulose production by activating the transcription of adrA .
In this study , we dem-onstrated for the first time that CsgD activates transcription of the promoter regions of adrA .
CsgD is also indirectly involved in cellulose production by activating transcription of adrA .
CsgD positively regulates the expression of adrA
CsgD is essential for , among other things , biofilm matrix production by inducing curli cellulose production , via adrA .
CsgD is essential for , among other things , biofilm matrix production by inducing curli production -LRB- via csgBAC regulation -RRB- , via adrA .
CsgD also positively controls expression of the diguanylate cyclase AdrA by direct binding to the promoter region upstream of adrA .
In contrast , CsgD activated adrA transcription only with RNA polymerase .
Cellulose production is initiated through CsgD-activated transcription of adrA ( 11 ) .
CsgD also activates expression of adrA , activating the cellulose biosynthetic operon bcsABZC .
CsgD also activates expression of adrA , increasing intracellular c-di-GMP levels .
Expression of the two biofilm-promoting factors is co-regulated , since the response regulator CsgD stimulates expression of both adrA .