To confirm that the observed changes in Pclass2 activity are a result of the modulation of FlhD4C2 autoregulation by FliT , we replaced the flhDC promoter with tetRA ) .
To confirm that the observed changes in Pclass2 activity are a result of the modulation of FlhD4C2 autoregulation by FliT , we replaced the flhDC promoter with the tetRA element from Tn10 ( PflhDC : ) .
Our data suggest that under certain environmental conditions a basal level of FlhD4C2 activity is maintained via a balance between external regulation of flhDC expression through , for example , FliT inhibition of FlhD4C2 .
The class 1 operon flhDC produces the multimeric master regulator of flagellar synthesis , FlhD4C2 .