These results suggest that SlyA controls expression of magainin 2 resistance determinants in addition to the ugtL gene , which appears to be the sole SlyA-regulated gene mediating polymyxin B resistance .
The polymyxin B susceptibility of the slyA mutant was the same as that exhibited by the ugtL mutant ( Fig. 5A ) and could be complemented to wild-type levels by a plasmid that expressed the ugtL gene from a heterologous promoter ( Fig. 5B ) , suggesting that ugtL is the only SlyA-regulated gene mediating polymyxin B resistance .
We propose that the SlyA proteins control ugtL transcription .
that both SlyA proteins bind to the ugtL promoter , suggesting that these two proteins use a feed-forward loop to control ugtL expression
These results demonstrate that the SlyA proteins bind to the ugtL promoter .
The SlyA proteins bind to the ugtL promoter .
These results suggest that SlyA controls expression of magainin 2 resistance determinants in addition to the ugtL gene .
We have established that transcription of polymyxin B resistance gene ugtL is controlled by the SlyA proteins in what appears to be a feedforward loop design .
The SlyA protein bound to a region located downstream from the ugtL transcription start site for a regulatory protein -LRB- Fig. 1A -RRB- .
Taken together with the identification of binding sites for both the SlyA in Fig. 2C , these results support the notion of a feedforward design in the regulation of ugtL transcription .
Taken together with the identification of binding sites for both the SlyA in the ugtL promoter , these results support the notion of a feedforward design in the regulation of ugtL transcription .
Model _ illustrating the feed-forward regulation of the ugtL gene by the SlyA proteins
Transcriptional control of the antimicrobial peptide resistance ugtL gene by SlyA regulatory proteins .
Transcriptional control of the antimicrobial peptide resistance ugtL gene by SlyA regulatory proteins .
Consistently , results from different laboratories showed that SlyA regulates a subset of PhoP-dependent genes , including ugtL , in Salmonella .
The S. Typhimurium ugtL are regulated by a similar mechanism , involving the coordinated actions of SlyA .
Transcriptional control of the antimicrobial peptide resistance ugtL gene by SlyA regulatory proteins .