In E. coli flagellar motility is negatively regulated by RcsB binding to the RcsAB box of the promoter of the flagellar master regulator flhDC .
RcsB is a previously described negative regulator of flhDC
We determined which flhDC promoter is regulated via RcsB using luxCDABE fusions to flhDC promoter mutants -LRB- Fig. 4C -RRB- .
gyrA was not shifted , indicating specific binding of RcsB to the flhDC promoter .
A control DNA fragment was not shifted , indicating specific binding of RcsB to the flhDC promoter .
A. Dose response curve of the binding of gray and His6-SUMO-RflM/RcsB complex ( black ) to the RcsB/RflM binding site of the flhDC promoter determined using MST .
A. Dose response curve of the binding of gray and His6-SUMO-RflM/RcsB complex ( black ) to the RcsB/RflM binding site of the flhDC promoter determined using microscale thermophoresis .
A. Dose response curve of the binding of RcsB protein and His6-SUMO-RflM/RcsB complex ( black ) to the RcsB/RflM binding site of the flhDC promoter determined using MST .
A. Dose response curve of the binding of RcsB protein and His6-SUMO-RflM/RcsB complex ( black ) to the RcsB/RflM binding site of the flhDC promoter determined using microscale thermophoresis .
In summary , our results demonstrate that RflM protein promotes RcsB target specificity for repression of flhDC Discussion Regulation of flagellar synthesis is controlled by the RcsCDB phosphorelay system in both S. enterica serovar Typhimurium .
In summary , our results demonstrate that RflM protein promotes RcsB target specificity for repression of flhDC Discussion Regulation of flagellar synthesis is controlled by the RcsCDB phosphorelay system in both S. enterica serovar Typhimurium .
In summary , our results demonstrate that RflM protein promotes RcsB target specificity for repression of flhDC Discussion Regulation of flagellar synthesis is controlled by the RcsCDB phosphorelay system in both E. coli .
In summary , our results demonstrate that RflM protein promotes RcsB target specificity for repression of flhDC Discussion Regulation of flagellar synthesis is controlled by the RcsCDB phosphorelay system in both E. coli .
In Salmonella , however , RcsB-mediated regulation of the flagellar master operon flhDC has been described to be independent from RcsA .
In the present study , we elucidated the mode-of-action of RcsB in regulation of flhDC .
the P1flhDC promoter _ resulting in high affinity binding of a RcsB-RflM heterodimer and repression of flhDC operon transcription
Phosphorylated RcsB is able to bind as homodimer with low affinity to the RcsB box in the flhDC promoter region .
RcsB also regulate cell motility by interacting with the flhDC promoter .