In the absence of these negative cis elements , ssrAB was no longer repressed by the global regulator H-NS .
that H-NS directly represses ssrAB
In this work , by combining biochemical strategies , we determined that H-NS represses the expression of ssrAB by initially binding to sequences located downstream of the promoter of this operon .
In this work , by combining genetic strategies , we determined that H-NS represses the expression of ssrAB by initially binding to sequences located downstream of the promoter of this operon .
cis elements required for the repression of ssrAB by H-NS .
H-NS represses ssrAB as well as many other Salmonella genes .
This indicates that the 302/478 region contains the cis elements necessary for the repression of ssrAB by H-NS .
the regulatory sequences _ required for the repression of ssrAB by H-NS
ssrAB-cat 69 indicates that when H-NS is inactivated , the 119/336 region is not able to mediate repression of ssrAB
ssrAB-cat 119 indicates that when H-NS is inactivated , the 119/336 region is not able to mediate repression of ssrAB
ssrABcat Fig. 4B indicates that when H-NS is inactivated , the 119/336 region is not able to mediate repression of ssrAB
ssrABcat 10 fusions indicates that when H-NS is inactivated , the 119/336 region is not able to mediate repression of ssrAB
a level of expression higher than that reached by the ssrAB-cat 302/478 fusion _ indicating that the 336/478 region mediates H-NS-indepen-dent repression of ssrAB
a level of expression higher than that reached by B _ indicating that the 336/478 region mediates H-NS-indepen-dent repression of ssrAB
a level of expression higher than that reached by Fig. 4A _ indicating that the 336/478 region mediates H-NS-indepen-dent repression of ssrAB
In the absence of the 119/336 region , the expression of ssrAB was no longer repressed by H-NS , whereas deletion of the sequence upstream of the position 55 did not affect the H-NS-mediated repression of FIG 6 HilD displaces H-NS from ssrAB .
In the absence of the 119/336 region , the expression of ssrAB was no longer repressed by H-NS , whereas deletion of the sequence upstream of the position 55 did not affect the H-NS-mediated repression of FIG 6 HilD displaces H-NS from ssrAB .
In the absence of the 119/336 region , the expression of ssrAB was no longer repressed by H-NS , whereas deletion of the sequence upstream of the position 55 did not affect the H-NS-mediated repression of ssrAB -LRB- Fig. 3 .
In the absence of the 119/336 region , the expression of ssrAB was no longer repressed by H-NS , whereas deletion of the sequence upstream of the position 55 did not affect the H-NS-mediated repression of ssrAB -LRB- Fig. 3 .
Taken together , these results indicate that H-NS represses the expression of ssrAB by acting on the 55/287 region .
the model in which H-NS represses the expression of ssrAB initially by binding by forming a nucleoprotein filament on the promoter
the model in which H-NS represses the expression of ssrAB initially by binding to two nucleation sites
Thus , these results indicate that the 336/478 region mediates H-NS-independent repression of ssrAB .
some of these H-NS binding sites may be unproductive for the repression of ssrAB
H-NS represses ssrAB by binding to multiple sites located in a region .