However , DNA sequence analysis suggest that the regulation of sirA by CRP is probably indirect .
However , gel mobility-shift experiments suggest that the regulation of sirA by CRP is probably indirect .
To test the hypothesis that the CRP binds directly to the sirA promoter , we used in-vitro gel mobility-shift assays .
that the regulation of sirA by CRP is indirect
Based on these observations , it seems unlikely that CRP directly controls sirA expression .
CRP functions as crp is necessary for SPI-1 genes expression by binding to its regulator sirA .
CRP functions as a transcriptional activator is necessary for SPI-1 genes expression by binding to its regulator sirA .