In the presence of an unknown environmental condition , HilD may activate invF expression .
If a condition exists in which hilA is repressed while invF expression is induced through HilD , it begs the question : Why produce InvF-dependent effectors in the absence of hilA expression when an intact type III secretion apparatus is not thought to be formed ?
Under inducing conditions , HilD also activates a second promoter far upstream of the invF translation start site , termed PinvF-2 -LRB- dotted arrow -RRB- .
We have evidence that HilD modestly activates invF expression independently of its effects on hilA , even in WT S. typhimurium .
Our studies show that HilD activate transcription of invF from a promoter .
We show that HilD can directly activate invF .
Figure 2B shows that in the hilA hilD mutant , HilD overproduction induces expression of invF , whereas expression of orgA remains unaffected .
Figure 2B shows that in the hilA hilD mutant , HilD overproduction induces expression of invF , whereas expression of prgK remains unaffected .
Figure 2B shows that in the hilA hilD mutant , HilD overproduction induces expression of invF , whereas expression of prgH remains unaffected .
We propose that HilD can independently activate invF expression .
HilD do not activate invF expression from the HilA-dependent invF promoter Previously , we had shown that HilA activates invF expression by direct interaction with sequences upstream of invF called the HilA box .
These results also explain why our invF-lacZ reporter plasmid , pVV448 , was not activated by HilD to the same extent as the chromosomal invF-lacZ fusion because pVV448 does not contain the HilD-and HilC-dependent +1 of invF .
It is possible that HilD , activates invF expression from a second promoter .
In order to address whether additional S. typhimuriumspecific genes are required for HilD to activate invF , we examined both pSA7 reporters in E. coli TOP-10 cells .
In order to address whether additional S. typhimuriumspecific genes are required for HilD to activate invF , we examined both pVV448 reporters in E. coli TOP-10 cells .
We found that HilD induce invF expression from pSA7 in E. coli , comparable to that .
We also found that HilD , activates invF expression from pVV448 , also comparable to that .
These results suggest that HilD might directly activate invF expression by binding to sequences upstream of invF .
These results suggest that HilD might directly activate invF expression by binding to sequences upstream of invF .
HilD bind to the invF promoter fragment in-vitro To examine whether HilD might directly activate the invF promoter by binding to sequences upstream of invF , we performed gel shift analyses .
HilD bind to the invF promoter fragment in-vitro To examine whether HilD might directly activate the invF promoter by binding to sequences upstream of invF , we performed gel shift analyses .
HilC directly activate invF expression by binding downstream of a HilD/C-dependent promoter Previous studies have shown that HilD can indirectly activate invF expression by derepressing hilA .
HilC directly activate invF expression by binding upstream of a HilD/C-dependent promoter Previous studies have shown that HilD can indirectly activate invF expression by derepressing hilA .
Discussion HilD directly activate invF expression by binding downstream of a HilD/C-dependent promoter Previous studies have shown that HilC can indirectly activate invF expression by derepressing hilA .
Discussion HilD directly activate invF expression by binding downstream of a HilD/C-dependent promoter Previous studies have shown that HilD can indirectly activate invF expression by derepressing hilA .
Discussion HilD directly activate invF expression by binding downstream of a HilD/C-dependent promoter Previous studies have shown that HilD can indirectly activate invF expression by derepressing hilA .
Discussion HilD directly activate invF expression by binding upstream of a HilD/C-dependent promoter Previous studies have shown that HilC can indirectly activate invF expression by derepressing hilA .
Discussion HilD directly activate invF expression by binding upstream of a HilD/C-dependent promoter Previous studies have shown that HilD can indirectly activate invF expression by derepressing hilA .
Discussion HilD directly activate invF expression by binding upstream of a HilD/C-dependent promoter Previous studies have shown that HilD can indirectly activate invF expression by derepressing hilA .
HilD appear to activate transcription of invF from a HilD/C-dependent start site .
Our studies also suggest that the downstream binding site is required for HilD to activate invF .
In fact , there is evidence that SipC HilD directly activate invF expression 723 can be secreted independently of SPI1 .
In fact , there is evidence that SipA HilD directly activate invF expression 723 can be secreted independently of SPI1 .
HilD are each capable of partially activating invF
HilD activate transcription from the p promoters in an apparently redundant invF sicA manner .
HilD activate transcription from the p promoters in an apparently redundant invF sicA manner .
In addition , HilD directly activate invF in non-HilA dependent manner .
HilD can activate expression of the invF and sicA/sip transcriptional units independently of HilA .
HilD , activate directly the expression of the invF operon , independently of HilA .
HilD all positively regulate invF .
invF is positively regulated by HilD through HilA
Moreover , HilD can activate invF expression in a aY .